Cytosine-to-uracil RNA editing is upregulated by pro-inflammatory stimulation of myeloid cells

Journal article

Hyomin Seo, Winston H. Cuddleston, Ting Fu, Elisa Navarro, M. Parks, Amanda Allan, Anastasia G. Efthymiou, Michael S. Breen, Xinshu Xiao, T. Raj, Jack Humphrey
bioRxiv, 2025

Semantic Scholar DOI PubMedCentral PubMed

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APA Click to copy
Seo, H., Cuddleston, W. H., Fu, T., Navarro, E., Parks, M., Allan, A., … Humphrey, J. (2025). Cytosine-to-uracil RNA editing is upregulated by pro-inflammatory stimulation of myeloid cells. BioRxiv.

Chicago/Turabian Click to copy
Seo, Hyomin, Winston H. Cuddleston, Ting Fu, Elisa Navarro, M. Parks, Amanda Allan, Anastasia G. Efthymiou, et al. “Cytosine-to-Uracil RNA Editing Is Upregulated by pro-Inflammatory Stimulation of Myeloid Cells.” bioRxiv (2025).

MLA Click to copy
Seo, Hyomin, et al. “Cytosine-to-Uracil RNA Editing Is Upregulated by pro-Inflammatory Stimulation of Myeloid Cells.” BioRxiv, 2025.

BibTeX Click to copy

@article{hyomin2025a,
  title = {Cytosine-to-uracil RNA editing is upregulated by pro-inflammatory stimulation of myeloid cells},
  year = {2025},
  journal = {bioRxiv},
  author = {Seo, Hyomin and Cuddleston, Winston H. and Fu, Ting and Navarro, Elisa and Parks, M. and Allan, Amanda and Efthymiou, Anastasia G. and Breen, Michael S. and Xiao, Xinshu and Raj, T. and Humphrey, Jack}
}

Abstract

Myeloid cells undergo large changes to their gene expression profile in response to inflammatory stimulation. This includes an increase in post-transcriptional modifications carried out by adenosine-to-inosine (A-to-I) and cytosine-to-uracil (C-to-U) RNA editing enzymes. However, the precise RNA editing targets altered by stimulation and the consequences of RNA editing on gene expression and the proteome have been understudied. We present a comprehensive RNA editing analysis of stimulated myeloid cells across three independent cohorts totalling 297 samples, including monocytes and IPS-derived microglia. We observed that C-to-U editing, while less abundant, has a higher effect size in response to stimulation than A-to-I, and has a greater potential to recode the proteome. We investigated the consequences of RNA editing on RNA stability and gene expression using in silico and in vitro reporter methods, and identified a recoding C-to-U site in ARSB that mimics a reported lysosomal storage disorder mutation.